Move beyond paper models and investigate DNA structure directly!
- Techniques: Micropipetting, fluorescence detection
- Topics: DNA structure, hydrogen bonds, fluorescence, biotechnology
- Time required: Can be completed in one or two 45-minute class periods
- Level: General high school through college
Discover the conditions that make double-stranded DNA denature into single strands, then anneal back together again. In this lab, students will use a fluorescent dye to investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. Inquiry-based investigations explore how DNA sequence, concentration, and pH affect base pairing through hydrogen bonds.
Required equipment (one per lab group):
- Micropipettes and tips
- Thermal cycler or heat block that can accommodate PCR tubes (ideal set up) or water bath and floaties (workable).
- P51™ Molecular Viewer or other blue light transilluminator (such as blueBox™ or blueGel™)
Storage notes:
- Reagents require refrigerator storage
- DNA binding dye must be protected from light
- Reagents are stable for 6 months when stored in the refrigerator and protected from light
Delivery information: The DNA Glow Lab™ kit contains reagents for 8 lab groups: Three DNA samples for analysis; DNA binding dye; Glow Lab Dilution Buffer 1; Glow Lab Dilution Buffer 2; Microtubes. Note: Reagents are compatible with P51™ Molecular Fluorescence Viewer or other blue light transilluminator (460-480 nm, e.g. blueBox™ or blueGel™).
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