Missy Baker is missing!
- Techniques: Micropipetting, PCR, gel electrophoresis
- Topics: Forensics, genes and disease, genotype to phenotype, cystic fibrosis, biotechnology
- Time required: Can be completed in two 45-minute class periods or one 90-minute class period
- Level: General high school through advanced high school
Missy Baker is missing and police have two suspects. Knowing that Missy Baker has cystic fibrosis, students will test DNA evidence from both suspects’ cars to see if it matches a person with a CFTR deletion. This lab provides an excellent overview of genetic technologies and connects their uses, from identification to genetic diagnosis, in an engaging and accessible format. With reliable results and a straightforward procedure, Missy Baker Missing is an excellent lab to introduce students to PCR and gel electrophoresis lab techniques.
Reagents and consumables supplied by user:
- Agarose (electrophoresis grade)
- DNA stain (e.g., GelGreen™)
- Gel electrophoresis buffer (e.g., 1X TBE)
- Distilled or deionized H₂O (to dilute buffer concentrate – not included in Lab Companion Kit)
- Microtubes
- PCR tubes
Required equipment:
- Micropipettes and tips
- Thermal cycler
- Gel electrophoresis and fluorescence visualization system
Storage notes:
- Reagents require freezer storage
- Reagents are stable for 12 months when stored in the freezer
Accessories information: The learning lab companion kit (catalog no. 470352-026) contains all the consumables (except water) needed for a class of 32 students. These reagents and consumables can also be sourced independently.
Delivery information: The miniPCR Crime Lab kit contains reagents for 8 lab groups: 2X EZ PCR Master Mix, Load-Ready™; 3X Crime Lab Primer Mix; 4 DNA samples to investigate, including controls; 100 bp DNA ladder, Load-Ready™. Note: Reagents are compatible with blueGel™ or other standard horizontal electrophoresis systems.
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